KMID : 0385520180310060232
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Analytical Science & Technology 2018 Volume.31 No. 6 p.232 ~ p.239
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The effects of human milk proteins on the proliferation of normal, cancer and cancer stem like cells
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Kang Nam-Mi
Cho Ssang-Goo Dayem Ahmed Abdal Lee Joo-Hyun Bae Seong-Phil Hahn Won-Ho Lee Jeong-Sang
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Abstract
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Human breast milk (HBM) provides neonates with indispensable nutrition. The present study evaluated the anti-cancer activity of diluted and pasteurized early HBM (< 6 weeks¡¯ lactation) on human breast cancer cell lines. The cell lines MCF7 and MDA-MB231 were exposed to 1% HBM from the 1st, 3rd, and 6th weeks of lactation and exhibited reduced proliferation rates. As controls, breast cell lines (293T and MCF-10A), breast cancer cell lines (MCF-7 and MDA-MB-231), and CD133hiCXCR4hiALDH1hi patient-derived human cancer stem-like cells (KUCSLCs) were treated with prominent milk proteins ¥â-casein, ¥ê-casein, and lactoferrin at varying doses (10, 50, and 100 ¥ìg) for 24 or 48 hrs. The impact of these proteins on cell proliferation was investigated. Breast cancer cell lines treated with ¥ê-casein and lactoferrin exhibited significantly reduced viability, in both a dose- and time-dependent manner. Interestingly, ¥ê-casein selectively impacted only cancer (but not normal breast) cell lines, particularly the more malignant cell line. However, ¥â-casein-exposed human breast cancer cell lines exhibited a significantly higher proliferation rate. Thus, ¥ê-casein and lactoferrin appear to exert selective anti-cancer activities. Further studies are warranted to determine the mechanisms underlying ¥ê-casein- and lactoferrin-mediated cancer cell-selective cytotoxic effects.
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KEYWORD
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human breast milk, beta-casein, kappa-casein, lactoferrin, Cell-proliferation, breast cancer cell
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